GSM1816446: NOMO1 3030 Bmal1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ARNTL
Cell type
Cell type Class
Blood
Cell type
NOMO-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
NOMO-1 cells
cell line
NOMO-1
cell type
MLL-AF9 leukemia
chip antibody
anti-Bmal1 (Abcam, ab3350, lot GR152555-3, 1mg/ml)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) was performed as previously described (Kowalczyk, 2012) with minor modifications. Specifically, cells were first fixed in 2mM EGS (Thermo Scientific) for 30 minutes or 1 hour, followed by 1% of formaldehyde for 30 or 15 minutes at room temperature respectively. Chromatin was sonicated to a size less than 500bp and then incubated overnight with appropriate antibodies. ChIP sequencing (ChIP-Seq) libraries were prepared through the process of DNA end-repair (Epicentre), A-base addition and adaptor ligation using indexed Illumina adaptors followed by enrichment PCR. All enzymatic steps are carried out using enzymes from New England Biolabs. Final libraries were pooled, size selected and sequenced on HiSeq2500 with 25 paired-end reads.